#3862 TGF-β1/ SMAD SIGNALLING IN ANCA ASSOCIATED GLOMERULONEPHRITIS

Abstract Background and Aims The transforming growth factor-β1 (TGF-β1) is a pleiotropic cytokine, with distinct roles both in fibrosis inflammation acts through the Smad signalling renal injury. We sought expression of TGF-β1/Smad ANCA associated glomerulonephritis (AAGN) we assessed this correlation injury at diagnosis as well progression. Method evaluated immunohistochemical TGF-β1, phosphorylated Smad3 (pSmad3) Smad7 semi-quantitatively quantitatively using computerized image analysis program different compartments 11 biopsies AAGN results were statistically analysed clinicopathological parameters. also used healthy controls disease other types Glomerulonephritis (GN). Results pSmad3 higher AAGN, compared to GN. glomerular presented preferable mesangial endothelial cells its intensity was correlated chronicity index [global glomerulosclerosis (p<0.001) interstitial (p = 0.03)] (Figure 1). reported that activity [cellular crescents 0.03), fibroid necrosis (p<0.001)] 2). TGF-β1 expressed peritubular capillaries around areas tubulitis more severe [higher creatinine 0.019) proteinuria 0.008)]. At last follow-up (median time 5 years) significant worst function 0.05) 0.005), while tended be beneficial maintenance eGFR 0.055). Conclusion activated